I desperately require help with this one. I have carried out isolation of dna from fungi and what I got was a band with a little shearing at around 23 kb. On looking up reference papers, it has been termed ‘nuclear dna’. I have few questions with respect to this:
-Nuclear dna of fungi is in the form of chromosomes whose size greater than kb, well into Mb I am sure. How can I get such a small sized bit of nuclear DNA?
-Could this 23 kb fragment be mitochondrial DNA, but isnt density gradient centrifugation required first to isolate mitochondria?
Im using the CTAB method for fungal DNA extraction
I would gladly accept any light shed on this matter.