I left my isoelectric focusing (IEF) equilibration buffer out at RT over the weekend. Is it still ok to use?
it should be fine.
You have there urea, rigth? That would crystalize in refrigerator anyway.
I’m not sure why, but these references say to not leave urea buffers at room temperature for extended periods of time.
http://onlinelibrary.wiley.com/doi/10.1 … 75.oth/pdf