3′ RACE

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    • #10931
      Autotroph
      Participant

      What are your experiences with 3′ RACE?

      It’s not working very well for me. I got some additional sequences, but when translated in all 6 frames, there’s multiple stop codons. It’s weird because the sequences are amplified from an mRNA library. Shouldn’t the sequences I get all be coding and therefore have almost no stop codons?

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