5′ LTR sequece

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    • #17849
      aguy
      Participant

      Hi,

      I want to check the 5′ LTR sequence of my plasmid. I will like to confirm that it is a hybrid 5′ LTR and not a wild type HIV-1 5′ LTR. Can someone pls guide me on how I can evaluate this?

      Best,
      John

    • #115347
      Helics
      Participant

      You may try to find comparable restriction endoneucleases for wild type 5’LTR.
      Hybrid 5’LTR generates different
      size RE digest products.

    • #115532
      feixiaorou
      Participant

      well, are there many different wild types out there? I mean, why not sequencing to find it out?

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