About Thiadiazuron(TDZ) and Phosphinothricin(PPT)

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    • #2029
      yamini
      Participant

      Could working on plant tissueculture and genetic transformation answer my doubt.
      I am trying to use TDZ to get transgenic shoots but my selection agent PPT seems to be sensitive. Even with very low PPT concentrations i am afailing to get shoots.
      Can anyone help?

      yamini

    • #30496
      mothorc
      Participant

      PPT in low content is a plant regulator, and there are some interaction between them but I can’t find any article about this problem
      If it is too sensitive, why don’t you use another hormone to regenerate shoot. TDZ just a group of both auxin and cytokinin.

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