Action of Gq protein

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    • #3088
      pitch
      Participant

      Hi,
      I don’t find references about the action of the Gq protein and my question is:
      The phospholipase C β(PLCβ) actives PIP2 and gives diacetylglycerol and IP3, this DAG actives the protein kinase C but I don’t know if the PKC follow the phosphorylation way of RAS and RAF or if the PKC follow another way to phosphotylate the MAPKs ❓
      Thanks.

    • #36170
      sdekivit
      Participant

      i thought PKC can directly phorylate MAPk

    • #36172
      Enzyme
      Participant
      quote pitch:

      Hi,
      I don’t find references about the action of the Gq protein and my question is:
      The phospholipase C β(PLCβ) actives PIP2 and gives diacetylglycerol and IP3, this DAG actives the protein kinase C but I don’t know if the PKC follow the phosphorylation way of RAS and RAF or if the PKC follow another way to phosphotylate the MAPKs ❓
      Thanks.

      Take a look to these books:

      – Berg J. M., Tymozcko J.L., Stryer, L. Biochemistry (5th edition). Freeman Publishers (2002).
      – Mathews C., Van Holde K.E. Biochemistry. Benjamin-Cummings Publishers.

      It is very good explained in those books. They were which I used when I studied the regulation by covalent modification of enzymes by phosphorilation and dephosphorilation.

      I hope the books will be useful for you :wink:. See ya!

    • #36174
      sdekivit
      Participant
      quote pitch:

      Hi,
      I don’t find references about the action of the Gq protein and my question is:
      The phospholipase C β(PLCβ) actives PIP2 and gives diacetylglycerol and IP3, this DAG actives the protein kinase C but I don’t know if the PKC follow the phosphorylation way of RAS and RAF or if the PKC follow another way to phosphotylate the MAPKs ❓
      Thanks.

      after reading this almost 10 times, i still don’t get you question. Do want to know the action of PKC on MAPk, or do you want to know if the activation of PKC is in the Ras-route ?

    • #36182
      pitch
      Participant

      Thanks Enzyme I’ve got the Stryer,5th edition that’s the greatest book for biochemestry but this is not a good book for cellular biology, and it doesn’t talk about MAPK,and if I am mistaken, gives me the chapter 😀 .
      Excuse me Sdekivit for my pitiful english but I would want to say that I never seen a mecanism where the PKC can directly phosphorylate MAPKs. In the way of cytokines as IL3 for exemple or the way of all the growth factor which I know PKC always activates RAF which can then phosphorylate MAPKs.
      Thanks for your attention 🙂

    • #36250
      Enzyme
      Participant
      quote pitch:

      Thanks Enzyme I’ve got the Stryer,5th edition that’s the greatest book for biochemestry but this is not a good book for cellular biology, and it doesn’t talk about MAPK,and if I am mistaken, gives me the chapter 😀 .
      Excuse me Sdekivit for my pitiful english but I would want to say that I never seen a mecanism where the PKC can directly phosphorylate MAPKs. In the way of cytokines as IL3 for exemple or the way of all the growth factor which I know PKC always activates RAF which can then phosphorylate MAPKs.
      Thanks for your attention 🙂

      Yes, Stryer is a great book of Biochemistry. I told you that there you can find information about phosphorilation and dephosphorilation (a type of regulation by covalent modification of enzymes).

      Take a look to this link:

      http://www.stanford.edu/group/ferrellla … /pub32.pdf

      Here it is a lot of information about MAPKs cascade. Also look at the bibliography (the authors used Stryer and many more great books).

      I hope this link is useful for you.

      See ya! 😉

    • #36330
      sdekivit
      Participant

      take a look in ‘Molecular Biology of the Cell’ by Alberts et al

      –> many signaltransduction pathways 🙂

    • #36336
      pitch
      Participant

      I asked to a resheacher who works in biology of development and he explain me, actually the PKC takes not always the pathway of RAF, but it is unable to directly phosphorylate the MAPKs, it takes another pathway a little bit more complicated.
      I thank to both for your help 😉

    • #36339
      Enzyme
      Participant

      OK, your welcome. Anyway, as in a few hours I’m returning to León (the city where I’m studying) and on Monday I start again the classes, I can ask your doubt to one of the professors of the department of Cellular Biology.

      I’ll tell you. See you! 😉

    • #36356
      sdekivit
      Participant
      quote pitch:

      I asked to a resheacher who works in biology of development and he explain me, actually the PKC takes not always the pathway of RAF, but it is unable to directly phosphorylate the MAPKs, it takes another pathway a little bit more complicated.
      I thank to both for your help 😉

      i still don’t understand: ‘the PKC takes not always the pathway of RAF’ 🙄

    • #36480
      pitch
      Participant

      sdekivit wrote:

      quote :

      i still don’t understand: ‘the PKC takes not always the pathway of RAF’

      Actually the PKC can sometimes phosphorylate RAF but it can active others proteins wich are going to phosphorylate MAPKs,it depend on the receptor and on the chain reaction involved. So there are many possibilities but the PKC never phosphorylate directly the MAPKs.
      I hope you understood now, but once again excuse me not to express me well in english 🙂

    • #36481
      sdekivit
      Participant

      ok, that’s what u meant 🙄

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