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    • #7624
      SororSaudade
      Participant

      Hello all!

      I’ve been trying to transform Agrobacterium tumefaciens, strain EHA105, by the freeze-thaw method. I’ve done it a few times and didn’t get any positive results, even with a liquid nitrogen shock before heating.
      I was wondering if anyone had problems with this and could give me any tips.

      Plus, is the triparental mating a better method to transform Agrobacterium? Has anyone tried these two methods?

      Thanks 😉

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