Biology Forum Molecular Biology Amino Acid Titration

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    • #11848
      micron66
      Participant

      Hello.

      I´m having problems understanding some of the key concepts of amino acid titration – I hop you can help me.

      As I understand it, the amino acid is at it´s fully protonated form when the solution is at it´s lowest pH value. How can this be, when the definition of a low pH value is a high concentration of H+? Why isn´t the amino acid fully deprotonated instead? And when the amino acid is at it´s highest pH value – why is it fully deprotonated instead of fully protonated? Now that the amino acid is fully deprotonated, the solution must be full of H+-ions, and therefore the pH should be very low.

      And one more thing: The amino acids that are negatively or positively charged at neutral pH – what happens to them during titration?

      Hope you can help me. Thank you.

    • #92938
      JackBean
      Participant

      That’s all about equilibria.
      Lets have some simple system with simple acid

      HA <-> H+ + A-

      if you add protons to the mixture, what will happen? You will have lot of protons, so the equilibrium will move to the left. And that is, why is amino acid protonated at low pH. The acidifying protons do not come from the amino acid (otherwise it would be at approx. neutral pH).
      The same applies at high pH, just reversed.

      About the charged AAs, they behave in accordance to they pK. If you have pH under it, the group will be protonated, if will be higher, the group will be deprotonated. For the same reasons as above 😉

    • #92955
      micron66
      Participant

      Thank you. I got it.

    • #99270
      elyateem
      Participant

      hi
      please…could anyone help me???
      i have a problem in calculating the net charge for the naa,aaa,baa
      i dont know where we put +.5,-.5,+1or -1
      plzzzzz help me

    • #99272
      JackBean
      Participant

      what is naa,aaa,baa? 🙄

      where does the stupid practice, not to write 0, comes from? 🙄

    • #99273
      elyateem
      Participant

      naa=neutral amino acid
      aaa=acidic amino acid
      baa=basic amino acid
      ……its not stupid

    • #99276
      JackBean
      Participant

      it is pretty stupid to write +.5, that’s nothing

      just look, what charged groups it have at the particular pH

    • #99280
      elyateem
      Participant

      iam not talking of ma own pocket
      its written like this in the book
      if you dont know wt iam talkin about so dont answer without say thats this is stupid question

    • #99281
      JackBean
      Participant

      I’m saying, it’s stupid to write like +.5, not that the question is stupid (not that it wouldn’t be…)

      I gave you the answer…

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