I have personally never used anti-fungal drugs in my cultures; good aseptic technique is well enough to keep your cultures clean. Unlike anti-microbial drugs, anti-fungal ones target eukaryotic cells, and thus the chances of side effects are much bigger with anti-fungal drugs. Could you give me an example of what kind of drugs people over there use?

I don’t know anyone around here who’d use anti-fungal drugs in their cultures, but then again, it probably depends on many factors, like what cells you use, what’s the aim of your experiment and so forth.

I’ve never heard of anti-fungal drugs in cell cultures either. Just an antibiotic in the growth medium and a good sterile technique were always enough to prevent infections…