I’m doing immunofluorescence in vitro and in vivo, and i found a new subcellular location for a protein with one antibody (which has been use in many publication). Pre-incubation with the immunogen peptide (blocking peptide) result in the loss of the signal. Overexpression of this protein in cells can be also seen. But using an other antibody shows a different pattern. Is it possible that one antibody (polyclonal) recognize two different proteins (preincubation with the peptide reveal the high affinity for my protein)?
Thanks for help.
In immunology, when talking about poly- vs. monoclonal antibodies, the specificity of the antibody can be the same antigen, just the polyclonal antibody recognizes many different epitopes and monoclonal just one.
Polyclonal antibodies against a single antigen can be produced in animals and they were the main antibodies used in immunology before hybridoma technology allowed the production of epitope-specific monoclonal antibodies.
Even today, polyclonal antibodies are sometimes used, for example when it is more important to bind the antigen (e.g. if you want to neutralize/eliminate it) than is to obtain an ultra-specific outocome.