Anybody knows how stable is ssDNA??

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    • #11441

      Hi guys,
      I’m preparing the probe for in situ hybridization. It’s the product from degenerated PCR so i guess it’s ssDNA. I labeled the probe with DIG, keep in 50%formamide and hybridization buffer with carrier DNA. I was so stupid that I left it overnight at my bench. Anybody has and information about how stable is ssDN at room temperature? especially in that buffer? Please help, my background in biochemistry is not that strong. Thanks a lot.

    • #91222

      i would say you may have degradation. A quick thing you can do is run a small quantity of it on a gel, and see if it is all the same size. If you get a smear, you had degradation.

    • #91425

      hydrogen bond

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