Aseptic conditions!

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    • #10743
      pinkypig
      Participant

      why is it important in cell culture to work under aseptic conditions when the growth medium already contains antibiotics?

      Any ideas?

    • #88447
      blcr11
      Participant

      Neither yeast (spores are everywhere) nor mycoplasma (you are probably carrying some on your hands right now) don’t care squat about antibiotics.

    • #88448
      MichaelXY
      Participant

      In what units are squats given.

    • #88451
      biohazard
      Participant

      Also, mycoplasma contamination is often extremily difficult to notice, especially during the early phase (so called cryptic contamination), during which you may easily spread it further or think that the results you get from your experiments are valid even though they’re compromised by mycoplasma. Antibiotics often slow down the growth of mycoplasma (but don’t kill it), so before you notice something is wrong, it’s everywhere.

      Yeast contamination, luckily, is much more easy to spot, so at least you know what cultures are affected and can ditch them.

    • #88487
      pinkypig
      Participant

      BUMP

    • #88493
      mith
      Participant

      why do you need to bump? seems pretty covered

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