BAC DNA purification

Viewing 0 reply threads
  • Author
    Posts
    • #18522
      kirupha
      Participant

      Hi all,

      I am trying to purify BAC DNA (106 kb) using Qiagen large construct kit. I am able to purify 5-10 ug of DNA but when I run on 0.8 % agarose gel I see a band near the well. After restriction digestion also I see this band but I can detect it with southern. But we need a clean prep to give it for pronuclear injection into mouse embryo. I tried phenol/chloroform to remove proteins but this seems to be genomic DNA. I have attached agarose gel picture (0.8 % gel) of undigested BAC DNA. Kindly help me to solve this problem

      Thanks,
      Kirupa


      Attachments:

Viewing 0 reply threads
  • You must be logged in to reply to this topic.