bacterial culture growth

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    • #16709
      siddharthsameer
      Participant

      Hello everyone
      I have one question , why do we measure the ph of Preculture.I made a a preculture of e.coli tof 10 for the competent cell, and i was asked to measure the ph of the preculture, and I had 3 precultre in different shake falsk, and the following pH I measured .
      shake flask- pH
      1 8,2
      2 8,4
      3 8,6
      I dont know the reason behind it, kindly let me know… I am stuck and can not find the answer..I would be highly obliged to you all. waiting for your response.
      with regards

    • #111945
      canalon
      Participant

      Never, ever did that. Sounds strange to me. Considering that they will be washed multiple times in plenty of different solutions and buffer any way…

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