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    • #8241
      Ammie
      Participant

      I have my insert in a topo plasmid and I want to change about five of the nt’s. What way is the best you think?

    • #76086
      canalon
      Participant

      It really depends where you want your mutations. Changing 5 successive (or very close) nucleotides and 5 nt spread over 1 kb are quite different things that will probably require different approaches.

    • #76562
      Ammie
      Participant

      I had 5 that were spread over around 6000kb.

      I decided to go with Multi QuikChange from Stratagene

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