Bioedit alignment

Viewing 1 reply thread
  • Author
    Posts
    • #7938
      jamiejlg
      Participant

      Hi- I’m using BioEdit for the first time to do a simple alignment but having trouble. It’s just a forward, reverse and respective primers.
      Do I need to complement my sequences or does bioedit (clustal, really) automatically do it?

      Thanks,
      Jamie

    • #74780
      leewengwah
      Participant

      Hi Jamie,

      BioEdit is a great tool. It’s quite brief on what you intend to do with your sequences. To design a PCR primer from the conserved region of sequences; you may perform the following steps:
      1) Import all your sequences into the BioEdit
      2) Perform a clustalw alignment
      3) Browse for the conserved region for the primer design

      Hope it helps.

Viewing 1 reply thread
  • You must be logged in to reply to this topic.