Buffer concentration

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    • #3532
      LaurieCate
      Participant

      The components of a typical buffer are as follows:

      100 mM Tris, pH 7.5
      50 mM NaCl
      10 mM MgCl2
      1 mM dithiothreitol (DTT)
      100 micrograms/milliliter BSA

      The above buffer is 1X (1-times concentrated). I need to write a recipe for a 10X-concentrated buffer for the enzyme buffer above.

      ____ mM Tris, pH 7.5
      ____ mM NaCl
      ____ mM MgCl2
      ____ mM dithiothreitol (DTT)
      ____ micrograms/milliliter BSA

      How would I go about solving this problem? Should certain components (such as DTT and BSA) be increased 10 fold? I am at a loss.

    • #40030
      sdekivit
      Participant
      quote LaurieCate:

      The components of a typical buffer are as follows:

      100 mM Tris, pH 7.5
      50 mM NaCl
      10 mM MgCl2
      1 mM dithiothreitol (DTT)
      100 micrograms/milliliter BSA

      The above buffer is 1X (1-times concentrated). I need to write a recipe for a 10X-concentrated buffer for the enzyme buffer above.

      ____ mM Tris, pH 7.5
      ____ mM NaCl
      ____ mM MgCl2
      ____ mM dithiothreitol (DTT)
      ____ micrograms/milliliter BSA

      How would I go about solving this problem? Should certain components (such as DTT and BSA) be increased 10 fold? I am at a loss.

      concentration muliplied by 10

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