February 7, 2006 at 3:02 pm #3532LaurieCateParticipant
The components of a typical buffer are as follows:
100 mM Tris, pH 7.5
50 mM NaCl
10 mM MgCl2
1 mM dithiothreitol (DTT)
100 micrograms/milliliter BSA
The above buffer is 1X (1-times concentrated). I need to write a recipe for a 10X-concentrated buffer for the enzyme buffer above.
____ mM Tris, pH 7.5
____ mM NaCl
____ mM MgCl2
____ mM dithiothreitol (DTT)
____ micrograms/milliliter BSA
How would I go about solving this problem? Should certain components (such as DTT and BSA) be increased 10 fold? I am at a loss.
February 7, 2006 at 3:12 pm #40030sdekivitParticipantquote LaurieCate:
concentration muliplied by 10
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