Buffer concentration - Biology Forum

last updated by

sdekivit 18 years, 9 months ago

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- February 7, 2006 at 3:02 pm #3532
  LaurieCate
  Participant
  The components of a typical buffer are as follows:
  100 mM Tris, pH 7.5
  50 mM NaCl
  10 mM MgCl2
  1 mM dithiothreitol (DTT)
  100 micrograms/milliliter BSA
  The above buffer is 1X (1-times concentrated). I need to write a recipe for a 10X-concentrated buffer for the enzyme buffer above.
  ____ mM Tris, pH 7.5
  ____ mM NaCl
  ____ mM MgCl2
  ____ mM dithiothreitol (DTT)
  ____ micrograms/milliliter BSA
  How would I go about solving this problem? Should certain components (such as DTT and BSA) be increased 10 fold? I am at a loss.
- February 7, 2006 at 3:12 pm #40030
  sdekivit
  Participant
  quote LaurieCate:
  The components of a typical buffer are as follows:
  100 mM Tris, pH 7.5
  50 mM NaCl
  10 mM MgCl2
  1 mM dithiothreitol (DTT)
  100 micrograms/milliliter BSA
  The above buffer is 1X (1-times concentrated). I need to write a recipe for a 10X-concentrated buffer for the enzyme buffer above.
  ____ mM Tris, pH 7.5
  ____ mM NaCl
  ____ mM MgCl2
  ____ mM dithiothreitol (DTT)
  ____ micrograms/milliliter BSA
  How would I go about solving this problem? Should certain components (such as DTT and BSA) be increased 10 fold? I am at a loss.
  concentration muliplied by 10
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