i’m looking for ideas and protocols how to measure Ca+2 conc. by using the fluorofore Flue-3 (single exitation wavelenght).
I understand the equation with Kd, Fmin, Fmax etc. but don’t know exactly how to do it: what conc. should I use for Ionomycin? and EGTA? and when does the EGTA should be added? before, during or after the loading? with the stimulant? after it?
thanks in advance!