September 23, 2020 at 6:56 pm #117422PankajParticipant
I need to calculate the Total % Adhered Bacteria for E.coli
I counted the CFUs and they were
566 at 10-2 dilution and 68 at 10-3 dilution
Now to calculate the Total % Adhered Bacteria I am using the following formula
Number of colonies adhered X dilution factor X 100/ Original inoculum
Also to calculate the original inoculum I am using the following formula:
(OD600 x 7.65) – 0.3 =? X 109 CFU/mL
For E.coli the ED600 value I got was 0.183
and after calculation the value I got was 1.09 X 109 CFU/mL
Now coming back to the calculation of Total % Adhered Bacteria
For 10-2 dilution I am doing this:
566 X 0.01 X 100/1.09 X 10^9 CFU/mL
Where 566 is the CFU count I counted after incubation
0.01 is the dilution factor and 1.09 X 10^9 is the original inoculum.
Please let me know if I am doing it right.
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