can amiRNA silence the agrobacterium?

Viewing 1 reply thread
  • Author
    Posts
    • #14179
      chuan
      Participant

      hello everyone,
      can amiRNA fragment kill the agrobacterium cell? I run into this problem recently. after i electroporate my amiRNA vectors into Lba4404 and c58 strains, they all do not grow. but the naked vector transformation grows very vell. so i need some suggestions about this amiRNA transformation. thanks! ❓

    • #102438
      JackBean
      Participant

      I guess that depends on the target gene. If you silence some important regulation factor, why couldn’t you kill the cells?

Viewing 1 reply thread
  • You must be logged in to reply to this topic.