I’m not sure where else to put this…doesn’t seem to fit anywhere else.
But if you have a lipid sample in a cuvette, I know that changing the concentration will affect the absorbance readings. But what about particle size? For example, I was working with two different lipids (phosphatidylcholine and phsophatidylglycerol). We were looking at how these proteins can package together in the presence of apolipoprotein — specifically, making nanodiscs.
If the lipids are able to pack more tightly together, would that result in a lower absorbance reading? Versus if you have lipids that aren’t able to pack more tightly, then wouldn’t you have more nanodiscs floating around? I’m not really sure.