Hello! I am new to this site, and this is my first time posting, so please excuse any errors I may make in the process. My question is if applying plant hormones (like IAA, Zeatin/Kinetin, ABA, and GA) topically to plant cell cultures will produce a quantitatively measurable result, or if the hormones will simply kill all the plant cells. I’m currently embarking on my first research project, and my idea will require the topical addition of hormone- laden sprays to different cell lines in order to determine their effects on the cells at large. I will also test if the resulting cell cultures will still be candidates for organogenesis, or if the addition of hormones may have stunted their capability by affecting some obscure feedback loop. At any rate, any advice or help you could offer would be greatly appreciated! 😀
how do you want to apply topically hormones to cell cultures? That means either callus or liquid cell culture, doesn’t it?
Anyway, the effect will be determined by amount of applied hormone, as well as by kind of hormone, or course (but also, whether will you use e.g. kinetin, trans-zeatin or cis-zeatin), and other factors.
JackBean is right. I just wanted to add that another major variable is the plant. Tomato and tobacco respond to the same hormone/dose/conditions in different manner. Different tissues/organs (stem, leaf, root) from the same plant respond in different manner (I said tissues/organs because plant biotechnologists refer to organs as tissues ("leaf tissue") as you cannot really use a single actual tissue in the experiment).
Also, you say you "will require the topical addition of hormone- laden sprays to different cell lines in order to determine their effects on the cells at large." How do you spray a cell line? They only exist in liquid cell culture as suspension cells…