Can we autoclave the blotting buffer?

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    • #8812
      ss_kalve
      Participant

      Can we autoclave the blotting buffer?

    • #79661
      canalon
      Participant

      Which buffer? with composittion we could tell if some components are sensitive to heat.

    • #79683
      ss_kalve
      Participant

      tris base and glycine

    • #79726
      canalon
      Participant

      As far as I know both can be autoclaved. But the question is : is it necessary to sterilize it?

    • #79792
      ss_kalve
      Participant

      I think so coz when i used autoclaved running buffer in electrophoresis it was giving good result than nonautoclaved one.so I’m thinking it should also give good result after autoclaving.

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