You trypsinize a T75 flask of CHO cells and resuspend your cells with media (15mL total). You determine your cell suspension contains 5 x 106 cells/ml. Howmany mLs of cells/media will you need to subculture a new flask containing:
a.50 mL of medium at a final concentration of 1.5 x 105 cells/mL?
b.140 mL of medium at a final concentration of 2.25 x 105 cells/mL?