for the cell lysis step as well? I am going to try both sonication and grinding techniques for cell lysis
2)Can I use Lowry assay if I use this 2D sample buffer for cell lysis. I know that Lowry assay is not reducing agent compatible, but the DTT concentration in this 2D buffer is 50mM. Do you think it will interfere with the Lowry assay?
3)I assume I can’t use Bradford assay here since I am using a detergent, CHAPS.