Chloroform and RNA isolation

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    • June 16, 2011 at 11:22 am #15091
      magnolia
      Participant

      Hello everyone!

      I need to isolate RNA using PureLink RNA Mini Kit, but the leaves that I’m doing the isolation from will be treated with Chloroform beforehand (CHCl3-dip). Will this interfere with the RNA isolation procedure?

      Thanks!

    • June 16, 2011 at 1:14 pm #105296
      magnolia
      Participant

      Actually, it’s dichloromethane dip (CH2Cl2). Sorry…

    • June 30, 2011 at 6:52 am #105430
      magicsiew
      Participant

      First of all, do you know what’s the purpose of CH2Cl2 treatment?

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