Hi everybody,

there isn’t any appropriate forum for this, so I will ask here.

I’m running some chromatographies with crude extracts from maize on Octyl Sep and High Q and because the extract is really dirty (just yeasterday, the Octyl Sep was totally black :lol:, now it looks a little better…), I need to clean the columns often and efficiently. So, I was looking to manuals, how to do that, but I have still two question (more like why, insted of how)

1)in Octyl Sep’s manual they write:

The question is – why should I use the detergent in acetic acid? I understand, that detergent will compete with the contaminants, so they will be washed out, so for that I need as hydrophobic enviroment as possible, but if I add HAc, that is contradictory, isn’t that?
Just another question came up. Because for the ethanol I need to use really low flow rate, I’m using 50% EtOH. Do you think, that’s enough for removing of the detergent?

2) in High Q’s manual there is:

They evokes for me, to use high salt buffer, than 30% acid and than NaCl in acid. So, you have like high salt -> no salt -> little salt and no acid -> high acid -> little acid. Wouldn’t be more logical to use first the NaCl in acid and than concentrated acid?

Thanks everybody 🙂