chromatography - Biology Forum

last updated by

sdekivit 17 years, 7 months ago

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- April 4, 2007 at 5:56 pm #7357
  logisticslord2
  Participant
  We did chromatography for nucleic acids. There are 3 Rf values calculated. They are .76, .44, and .21. The values given for RNA=.71 and DNA=.39. How do we explain all three of these then? Does it have to do with what bases are present? Help please…if you have a link that’s excellent but if not that’s cool too..
- April 7, 2007 at 10:02 am #70933
  sdekivit
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  it can be a slight mistake in you mobiule phase (slightly more polar, since the Rf-values are higher if you use normal phase).
  Baed on your results, i would say Rf = 0,76 = DNA and Rf = 0,44 = RNA. Probably the other fraction is unremoved denatured protein.
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