January 6, 2007 at 5:40 pm #6695Miss_MeParticipant
Colchicine is a chemical which has an influence on cell division by obstructing the production of spindles and so chromosomes cannot move to the opposite poles.
I would like to know if there are any other chemicals present that have the same effect as Colchicine?
Is there another chemical which has the same overall effect as Colchicine but acts on the cell in a different way?
January 6, 2007 at 7:36 pm #64798sdekivitParticipant
well the mitotic spindle is formed by microtubuli, so maybe you can search for chemical that block microtubulus-polymerisation.
January 6, 2007 at 11:01 pm #64814MrMisteryParticipant
i know bromnaphtalen does the same thing, but no idea about the mechanism…
November 10, 2007 at 9:48 am #77633MrMisteryParticipant
November 11, 2007 at 8:36 am #77643JammerzParticipant
There are other microtubule depolymerizing drugs similar to colchicine, like nocodazole and colcemid, that bind free tubulin in a cell and prevent them from adding onto microtubules.
I also know of a drug called Brefeldin that interferes with anterograde transport from the endoplasmic reticulum to the golgi apparatus but I’m not sure of the mechanism (I would assume it disrupts kinesin/microtubule interactions in some way).
November 16, 2007 at 6:55 pm #77864
i found it on a genetic paper.
colchicine is a substance wich produces an abnormal mitosis (called c-mitosis), it annules the chromatic bobbin formation (wich is very important in the sister chromatides separation on anaphase) and also as a consequence, annules the cellular partition too.
with colchicine you can create the polyploidies.
and yes, there is another chemical compounds.
You can use N2O (nitrous oxide) on flowers just polinized.
And there is another methods to do the same thing that colchicine do.
November 17, 2007 at 3:09 pm #77935quote kclo4x:
When you make a polyploid for example with colchicine, you’ve to protect the root area because if it is in contact with the compound it will death and it’ll be an nonfunctional plant (root cells will die).
So, make your own conclutions.
Now, i’m gonna explain how to use N2O on the plant.
Treatments with nitrous oxide in low pressure on flowers just polinized during 10-15 hours. It penetrates easily in tissues and also has easy disappearance, but with annoying application (you have to do it on special containers :P).
It is also dangerous because of the gas’ toxicity.
The N2O reduces the typical colchicine’s chimeras.
pd: my english is a little poor so pardon me please jeje :P.
November 18, 2007 at 6:19 pm #77992quote kclo4x:
yes, one step in the method with colchicine says: cover the roots from the colchicine.
Xclo4x, try to maintain to me informed about the lettuce please, i’d like to know about it.
November 22, 2007 at 4:02 am #78271quote kclo4x:
🙂 nice !
i just hope that the growing stage that your lettuces are experimenting now is not the growing stage powered by the endosperm resources.
keep it up dude !
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