competitive inhibitors

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    • #14366
      Hudson
      Participant

      You are observing an enzyme-driven reaction. To the reaction mixture, you add a chemical, X, that inhibits the reaction. You suspect that X is a competitve inhibitor. What 2 pieces of evidence might lead you to this conculsion?

      I’m a little lost on this question. I compared competitive inhibitors with noncompetitive inhibitors, but im not sure if i’m on the right track. I thought maybe one piece of evidence could be that the reaction was occuring at the active site when it stopped because competitive inhibitors do their work there, but how would one know if thats wehre the reaction was taking place. can you see a reaction take place and where?? . Or would i have to say that i was observing a plotting a Vmax graph and the results indicated it was competitive?

      Please help 🙁

    • #103135
      JackBean
      Participant

      sure, plot is a good indication.The other could be structure similar to substrate or cofactor, which might be replaced and thus the reaction inhibited

    • #103300
      sconov
      Participant

      try to add more the substrate. if the substrate is higher than the competitive inhibitor, the competitive inhibitor will lose its ability to use active site of enzyme.

    • #103301
      JackBean
      Participant

      that’s not that simple. That depends on both KM of the substrate and Ki of the inhibitor

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