Biology Forum Molecular Biology Concentrating RNA

2 voices
1 reply
  • Author
    Posts
    • #13306
      Lakia
      Participant

      Hi all,

      I have a few samples of RNA that I want to use for RACE PCR. The concentration in the samples is too low (around 30-60ng/microlitre) to make RACE cDNA at the moment but I think I can get a high enough concentration if I pool my samples (I have about 30 microlitres from several RNA extractions) and concentrate them down to about 8 microlitres. I am just wondering what the best method for concentrating the RNA. We have a speed vac in the building, but I think it only runs at ambient temperature at the best so I am a bit worried about degrading the RNA. Ive also considered ethanol precipitation but I’m worried about losing some RNA through that method. Ive had another suggestion that I can leave the sample in a fume hood and just let the water evaporate but that sounds a bit risky also. What is the safest method to concentrate the RNA?

    • #99721
      JackBean
      Participant

      you can use either some NA binding columns or ethanol precipitation. Evaporating water in fume hood would take quite long time… And you couldn’t control much the volume 😉

You must be logged in to reply to this topic.

Members