constuction of a full length gene

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    • #11294
      shivakumar
      Participant

      hi everybody
      how to construct a full length gene from partial length cDNAs available from sutracted cDNA libraries. What are the techniques involved in it. And suggest me some related websites, plz.

      thanks a lot in advance

    • #90481
      MrMistery
      Participant

      well there might be a smarter way to do this, but you could just PCR them up with ends that can be cut by different restriction enzymes, so that the site on the end of one partial copy matches the site at the beginning of the next one. Then cut them, column-purify, and ligate.

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