Denatured protein

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    • #15736
      Maatomaat
      Participant

      Is there any possible way to fix denatured protein?

    • #108390
      JackBean
      Participant

      how is it denatured? You can try to dissolve it in urea and then decrease slowly concentration of urea, that should lead to folding.

    • #108396
      Maatomaat
      Participant
      quote JackBean:

      how is it denatured? You can try to dissolve it in urea and then decrease slowly concentration of urea, that should lead to folding.

      For example Acidic environment cause it.
      urea what you said, is one of the denaturant factors. How and why can it be used at folding it?

    • #108408
      JackBean
      Participant

      that was just idea, since I know, that proteins are sometimes extracted with the use of urea and then refolded during dialysis. But with the acidic environment it should work as well. Slowly increasing pH and the protein could refold.

    • #108428
      want2be
      Participant

      i asked the same question to my bio teacher, and apparently there is no possibility that denatured protein can be fixed, unless someone have found something to fix the denatured protein

    • #108441
      Cat
      Participant
      quote want2be:

      i asked the same question to my bio teacher, and apparently there is no possibility that denatured protein can be fixed, unless someone have found something to fix the denatured protein

      Incorrect.
      It will depend on the protein. If it is capable of spontaneous folding, it will probably refold when environment returns to normal. If it needs chaperones to help it fold, it would not refold. Ex: water soluble proteins are more likely to refold than membrane proteins.

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