Yes, blood plasma. I need to infuse radiolabelled glucose in vivo and measure hepatic glucose production. I am just not sure the best way to deproteinize the blood to get the infused glucose vs. blood glucose values?
Hello! Here is a protocol currently used by a core facility for the deproteinization of small volumes of mouse plasma for measurement of plasma radiolabeled glucose:
1) Add 10 uL of plasma into 20 uL of dd water.
2) Add 25 uL of BaOH (0.3N from Sigma B4059) and then add 25 uL of ZnSO4 (0.3N from Sigma Z2876).
3) Vortex and spin at 12,000 rpm for 10 minutes.
4) Collect 25 uL in duplicate and then add to 3-5 mL of scintillation fluid for counting.
I hope this helps someone even though I’m responding to a 3-year old question. If anyone finds this helpful please let me know!