Hi all

I would like to know if anyone has ever worked with Desferrioxamine (=desferal, DFO, DFX). Under hypoxic conditions, this substance activates HIF-1 and so EPO is produced.

The thing is that we have to inject genetically engineered C2C12 myoblasts treated with desferal into mice (I’ve read this has to be done intramuscularly) but I can’t find how to do so correctly.

I read that it can be dissolved in PBS but I don’t know how many injections nor how regularly I should inject. Could the dosis be 200-300mg/kg wt more or less? (I read this in the literature).

Besides, I am not used to doing intramuscular administrations in mice, so I would be very grateful if anyone could give me a hand with this issue as well. – webpages images??

I have read papers in which DFO has been used, but was administered alone (and I am interested in injecting cells treated with DFO, not DFO alone")

Thanks very much beforehand
Regards,
Ainhoa