Designing Primer for over lapping PCR

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      Rezaulbiox
      Participant

      Hello everyone,
      I am trying to amplify a little bit long DNA of 6.72kb. I designed primer within 100 bp upstream and downstream of this DNA but failed to get the DNA by PCR. Some one suggested me to amplify the DNA by overlapping PCR. I do not know how to design the primer for overlapping PCR. I usually used Primer Premier 6 as primer designing software.

      You are highly appreciated to give me some guidelines to design overlapping primer by using Primer Premier 6.

      Thanking you
      Khan

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