Hi,
I’m just doing a piece of work at the moment and I’m a little stuck. I’ve got a DNA ligase sequence and I’ve created primers for it to mutate two residues but now I have to design two outer primers to clone the gene into a pET expression vector and I have no idea how!
I’ve pretty much been staring at a blank page all day but I think I might have just had a breakthrough thanks to trusty wikipedia, on the site directed mutagenesis wiki page it says "Variations employ three or four oligionucleotides, two of which may be non-mutagenic oligonucleotides that cover two convenient restriction sites and generate a fragment that can be digested and ligated into a plasmid, while the mutagenic oligonucleotide may be complementary to a location within that fragment well away from any convenient restriction site.
Am I right in saying that what I should be doing is creating another pair of primers, one upstream and one downstream of my current primers, which cover a restriction site which can be cut to create a sticky end which I can use to insert my protein into a pet vector?
Thanks!
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