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    • #17287
      LittleMike
      Participant

      Hi,
      In the lab,I use fluorescence spectrometer for the ratiometric fluorescence measurement which needs to measure optical intensity at two different wavelenghts.
      However, the fluorescence spectrometer is expensive and huge. I am wondering how to build a portable device for the ratiometric fluorescence measurement?
      Many thanks!

    • #113646
      jonmoulton
      Participant

      If you don’t mind losing the flexibility of scanning the spectrum or rapidly changing ex/em bands, you would build a 2-band fluorescence colorimeter. You’d need two emission filters and one or two excitation filters, arranged around your cuvette so that the excitation and emission light paths intersect at a 90 degree angle in the sample. Of course, you’d need an excitation source and either two photodetectors or a means of manually switching the emission filter, plus the electronics to capture the photodetector output and do the ratiomatric comparison.

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