Difference between? Complementaton and Recombination

Viewing 7 reply threads
  • Author
    Posts
    • #12877
      doctobe
      Participant

      In light of the Complementation test performed by Benzer on T4 phages, what is the difference between complementation and recombination?
      (Can you have complementation and not have recombination, can you have complementation and have recombination?

    • #98033
      JackBean
      Participant

      I think, that complementation and recombination has nothing to do basically.
      Recombination is exchange of genetic material between two DNA molecules (chromosomes, plasmids etc.), whereas complementation is process, when you add some gene to compensate for missing endogennous gene.
      E.g. bacteria need some osmosensor to grow, so when you have knock-out of it, they are not able to survive (maybe only on osmotic media, not sure now), but when you add some other histidine kinase and it’s signal, they are viable. Apparently, because the new HK supplemented bacteria with "osmosensor", but there does not have to be recombination (it can stay on plasmid).

    • #98098
      doctobe
      Participant
      quote JackBean:

      I think, that complementation and recombination has nothing to do basically.
      Recombination is exchange of genetic material between two DNA molecules (chromosomes, plasmids etc.), whereas complementation is process, when you add some gene to compensate for missing endogennous gene.
      E.g. bacteria need some osmosensor to grow, so when you have knock-out of it, they are not able to survive (maybe only on osmotic media, not sure now), but when you add some other histidine kinase and it’s signal, they are viable. Apparently, because the new HK supplemented bacteria with “osmosensor”, but there does not have to be recombination (it can stay on plasmid).

      "How" did HK do this? Did you guess about the HK doing that? How did you prove(or how was it proven) that it was supplemented with the osmosensor"?

    • #98107
      JackBean
      Participant

      they are quite similar, so they can supplement each other. This system is commonly used in such situations.
      There is system in both E.coli and Saccharomyces

    • #98117
      doctobe
      Participant
      quote JackBean:

      they are quite similar, so they can supplement each other. This system is commonly used in such situations.
      There is system in both E.coli and Saccharomyces

      it is the Ecoli system that was given as an example in class. Trying to understand that one. with the B strain K strain.

    • #98134
      JackBean
      Participant

      that is something different. I’m talking about histidine kinases, which work as receptors and thus, if you have knock-out of one of them, you can supplement it with plant histidine kinase

    • #98139
      doctobe
      Participant
      quote JackBean:

      that is something different. I’m talking about histidine kinases, which work as receptors and thus, if you have knock-out of one of them, you can supplement it with plant histidine kinase

      I went to my teacher yesterday and it seems I was remembering examples versus concepts. I though Ecoli was used as an example but it turns it out that is was specific to Ecoli and not to every system.
      NOw trying to unlearn somethings and regroup my thoughts. Thanks for replying.

    • #98153
      JackBean
      Participant

      with the T4 phages you can probably use only E.coli, but I gave you another example, from my work, where can be used either E.coli or yeast

Viewing 7 reply threads
  • You must be logged in to reply to this topic.