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    • #14462
      chengieteo
      Participant

      Hi all,

      I’m quite new in Molecular biology and wish to know more. I saw this two question not very sure how we can do it.

      How can we differentially label (make different) DNA and RNA?
      How can we differentially label (make different) DNA/RNA and protein?

      Hope that someone can assist me in this. Thanks in advance =)

      Regards,
      Cheng

    • #103412
      JackBean
      Participant

      for labeling NAs you can use e.g. Cy3 and Cy5 or plenty fluorescent colors used in qPCR such as TaqMan and many others.

      And I think that Cy3 and Cy5 can be used even for proteins. For some other labels look for differential 2D electrophoresis.

    • #103443
      peyem
      Participant

      For RNA and DNA, is it the uracil in RNA and the thymine in DNA that will produce different fluorescent colours?

    • #103462
      canalon
      Participant

      There are countless fluorescent markers who have different physical properties and are able to give different colors.
      T and U are not fluorescent by themselves, but if a fluorescent moiety is attached to them (obviously with different spectral properties) they will be easy to separate.

      Handy list of different fluorophores here:
      http://en.wikipedia.org/wiki/Fluorophore

      One thing they have in common though is that they are quite expensive.

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