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    • #12030
      carbonp
      Participant

      Take 5 small glass test tubes (1-5) and put 1200 μl of distilled water in each.
      Now add 300 μl of 10 μM atropine to Tube 1, mix well and transfer 300 μl to Tube 2.
      Mix well and transfer 300 μl to Tube 3.
      Repeat up to Tube 5.
      Calculate the atropine concentration in each tube.

      my question is
      For first test tube,
      C1 = 10 micromolar
      V1 = 300 microliter
      C2 = we are finding
      V2 = 1200 or 1500 microliter?????????????????

    • #93806
      mith
      Participant

      you started with 1200, and you added 300ul fluid. for the purpose of the calculation you have 1500, however by distributing the mixed contents, your final volume in the tube is 1200.

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