Hi, I am using QIAamp kit to extract DNA from Acanthamoeba culture. However, I just able to extract about 40ng/ul of concentration only when measured by NanoQuant. Is this concentration normal for Acanthamoeba ? This is because previously extraction from other sample can obtain high concentration up to 300ng/ul. Or is there any pre-treatment required to be done to the sample before using this Qiagen kit? I am sure there is no other step stated in Qiagen handbook, just that any other step that is specifically to acanthamoeba?
I have done cell count, using 3.2million culture, but i just pipetted 1ml and do the extraction with the modified protocol. Add in proteinase K initial, incubate at 56’c for 10min. Yield positive results.