i ever used Intelligene’s lysis buffer. Its’ protocol is very easy. you can put your sample tissue in lysis buffer –>grind it well–>incubate at 95 degree celcius for 15 min–>centrifuge–>pipette the supernatant to 95% ethanol–>centrifuge–>dry pellet and dissolve with TE buffer or DI water.
After i check DNA quality with spectrophotometry and gel electrophoresis, i’s ok–>there are protein interfere a little. I would like to know what is the component of this lysis buffer. Who can guess? please tell me.thank you. 😀
Geez, I had no idea what you guys were talking about, maybe after next semesters Micro Bio class, but that Pro K sounds like a good agent for jellyfish stings. Sounds better than the alternative, which I am sure you have heard of.