I’m constructing a new vector and before starting ligation I measured dna purity of my gene. The dna purity that I measured is 17. More more than 1.8 😕 What does this mean, is my dna is so pure from proteins etc 😀 I measured this three times, it is unable to understand for me. Could anyone explain me this?
Thanks for yor help
Yes I’m sure that it is 17 not 1.7. The spectrophotometer calculates automaticly when I enter my dilution ratio. I used spectro lots of times and that is the first time ı see a value like this. But ı make dna cleaning and concentrating prosedure and forget my dna sample 5 or 7 minutes in the final step(elution step). Is this the matter I really don’t undestand??
I also want to ask another question like this problem. Today I purified DNA from pellets. Then I read their purity and concentrations in spectrophotometer. The purities range between 1,8-1,9. Really good and concentrations are good also. Then I digest this samples with an enzyme and look if it digest, the digestion was perfect. I cut the bands that I want and the problem starts at this point: I made gel extraction and now the purities range between 3,5-5 . The concentrations are 5 mikrogram/ml. I’m so unhappy that you can’t guess after the perfect purity and concentration from first step. Is it the agarose that wastes my samples? What will I do?