DNA restriction sites/fragments calculation?

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    • #18277

      What types of DNA fragments would result if you were to cut the plasmid shown with both restriction enzymes, EcoR1 and BamH1?

      a. 1/2 20 kb, 1/4 30 kb, 1/4 10 kb
      b. 1/2 20 kb, 1/2 60 kb
      c. 1/2 30 kb, 1/2 50 kb
      d. All 80 kb

      I have identified the answer as being a.

      My reasoning is this:

      Distance between EcoR1 (20kb) and BamH1 (40kb) =20 kb

      Distance between BamH1 (40kb) and BamH1(70kb)=30kb

      Distance between BamH1 (70kb) and EcoR1 (80kb) =10kb

      What I am confused by though, is the 1/2 and 1/4 component of the answer given. Can anyone give me a breakdown of that please?

    • #116033

      Your image did not show correctly, could you post again?

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