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    • #17586
      ophelia15
      Participant

      Hello everyone, I was wondering if complementary DNA is a copy of the entire messenger RNA or only a copy of the coding parts ?
      I’m really confused…
      For example, if I have the mRNA of a particular gene with some non-coding parts, will the cDNA be exactly the same as the mRNA or it will contain only the coding parts ?

    • #114639
      jonmoulton
      Participant

      cDNA can contain 5′ and/or 3′ UTR sequence but not introns.

    • #114640
      JackBean
      Participant

      of course it will be reverse complement, but considering the length, it will cover whole mRNA (at least in theory, depending on the primers etc.)

    • #114641
      JackBean
      Participant

      which reminds me, if you use gene-specific primers, you will only amplify the region you designed the primers for! Which can, but probably doesn’t include UTRs

    • #114647
      jonmoulton
      Participant

      Right – another technique, like RACE, is usually needed to extend the sequence across UTRs. If the primers are designed against genomic sequence, they can include UTR sequence, but are unlikely to capture the entire UTRs.

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