- October 19, 2013 at 10:25 pm #17586ophelia15Participant
Hello everyone, I was wondering if complementary DNA is a copy of the entire messenger RNA or only a copy of the coding parts ?
I’m really confused…
For example, if I have the mRNA of a particular gene with some non-coding parts, will the cDNA be exactly the same as the mRNA or it will contain only the coding parts ?
- October 28, 2013 at 3:46 pm #114639jonmoultonParticipant
cDNA can contain 5′ and/or 3′ UTR sequence but not introns.
- October 28, 2013 at 5:27 pm #114640JackBeanParticipant
of course it will be reverse complement, but considering the length, it will cover whole mRNA (at least in theory, depending on the primers etc.)
- October 28, 2013 at 5:28 pm #114641JackBeanParticipant
which reminds me, if you use gene-specific primers, you will only amplify the region you designed the primers for! Which can, but probably doesn’t include UTRs
- October 29, 2013 at 3:14 pm #114647jonmoultonParticipant
Right – another technique, like RACE, is usually needed to extend the sequence across UTRs. If the primers are designed against genomic sequence, they can include UTR sequence, but are unlikely to capture the entire UTRs.
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