dot blot–protoplasts

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    • #15920
      biology_06er
      Participant

      A question regarding protoplasts in a dot blot..my protein of interest is in the protoplast which I has isolated and I have been told to do a titration/dilution of it…so I was told do 4x 10 fold diltuin…does this mean if I started with 1:10 my next wld be 1:100, 1:1000, 1:10000/—is this right? Is there a “certain” starting dilution I should start with?

      Regards,
      B_06er

    • #108997
      JackBean
      Participant

      the first one will be probably 1 : 1 😉

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