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    • #15847
      Kiki11
      Participant

      Hi,

      I am trying to generate a minimal media for the E.coli strain with the following genotype
      F'[lacIq Tn10(tetR)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG
      with the addition of Leucine I have managed to grow them, but the yield is anything but great. I am a little confused about the combination of araD139 and Δ(ara-leu) in the genotype.

      araD139 means, that a mutation in L-ribulose-phosphate 4-epimerase blocks arabinose metabolism and
      Δ(ara-leu) means, that the ability to built arabinose (and leucine) is deleted.

      Can somebody help me and tell me if or if not my E.colis need arabinose? And/or if they are missing something else, please?

      Thank you
      Kiki

    • #108733
      JackBean
      Participant

      just try to add the arabinose and you will see 😉

    • #112890
      kdean
      Participant

      I believe that the ∆(ara, leu) simply means that your strain of bacteria cannot catabolize arabinose or leucine.
      http://en.wikipedia.org/wiki/Bacterial_ … menclature

    • #112892
      JackBean
      Participant

      catabolise or synthesise?

    • #112971
      citroenboom
      Participant

      According to openwetware it blocks metabolism.
      I am using TOP10 and it has the same deletion. My cells do happily grow on M9 or the minimal medium of our group with leucine. DH5a doesn’t have it I think.

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