E.coli MC4100 thi- genotype

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      julian
      Participant

      I’m trying to create a pspA reporter plasmid by linking the psp promoter using the thi- genotype of E.coli MC4100 as an auxotrophic marker, however I’m having ‘a little’ trouble finding out which gene has actually been deleted or mutated so that I can incorporate it into my plasmid.
      Does anyone out there know what gene the thi- in E.coli MC4100 actually is?

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