Hi, I have cloned out a gene that without annotation of my particular species in GenBank, when I blast the sequences, able to find the same gene from related species. But the e value are 0.074 and 0.006 respectively. By just analyzing from this data, can I conclude I have successfully clone out the gene? I think these e values are high, but since they are totally different species, so are these e value acceptable? Or is there any parameter of e value for different species?